Izinto ezikhanga amandla aphezuluI-DMPTfuthiI-DMTziyizinto ezintsha nezisebenzayo ezikhanga izilwane zasemanzini. Kulolu cwaningo, izinto ezikhanga kakhuluI-DMPTfuthiI-DMTkwenezelwa ekudleni kwe-carp ukuze kuhlolwe imiphumela yalezi zinto ezimbili ezikhangayo ekudleni kwe-carp kanye nokukhuthazwa kokukhula. Imiphumela ibonise ukuthi ukwengezwa kwezizinto ezikhangayo ezinamandla aphezuluI-DMPTfuthiI-DMTukudla kwandise kakhulu imvamisa yokuluma kwezinhlanzi ezihlolwayo futhi kwaba nomphumela obalulekile wokudla; ​​Ngesikhathi esifanayo, ukwengezwa kwezinga elihlukene lezinto ezidonsela amandla aphezulu.I-DMPTfuthiI-DMTUkwanda kwesisindo sezinhlanzi ezihlolwayo kwandise kakhulu izinga lokukhuluphala, izinga lokukhula elithile, kanye nezinga lokusinda kwezinhlanzi ezihlolwayo, kuyilapho i-coefficient yokudla yehla kakhulu. Imiphumela yocwaningo ikhombisa nokuthiI-DMPTinethonya elibaluleke kakhulu ekudonseni nasekukhuthazeni ukukhula kwe-carp uma kuqhathaniswa ne-I-DMT.

Isithako sokudla kwezilwane emanzini siyisengezo esingenamsoco. Ukwengeza izithako zokudla ezinhlanzini kungakhuthaza ukudla kwazo ngempumelelo, kwandise ukudla kwazo, kunciphise ukudla okusele emanzini, ngaleyo ndlela kunciphise ukungcola ezindaweni zamanzi ezifuywayo.I-DMPTfuthiI-DMTziyizinto ezisebenzayo ezitholakala kabanzi ezintweni eziphilayo zasolwandle, ezisebenza njengabanikeli be-methyl abaphumelelayo kanye nabalawuli ababalulekile bengcindezi ye-osmotic. Ziphinde zibe nemiphumela ebalulekile yokudla nokukhuthaza ukukhula ezilwaneni zasemanzini.

Ngemva kokwenza izifundo ezifanele ezilwaneni zasemanzini njenge-crucian carp, i-red snapper, i-goldfish, kanye ne-spotted shrimp, abacwaningi baseJapan bathole ukuthiI-DMPTfuthiI-DMTinemiphumela emihle yokukhanga ezinhlanzini zamanzi ahlanzekile nezasolwandle, izilwane ezinamagobolondo, kanye nezinhlanzi ezinamagobolondo. Ukwengeza amazinga aphansi ezikhanga ezinamandla aphezuluI-DMPTfuthiI-DMTekudleni kungasheshisa kakhulu ukondla nokukhula kwezinhlanzi ezahlukahlukene zamanzi ahlanzekile nezasolwandle. Kulolu cwaningo, izinto ezikhanga kakhuluI-DMPTfuthiI-DMTkwenezelwa ekudleni kwe-carp ukuze kufundwe ngemiphumela yako ekudleni kwe-carp kanye nokukhuthazwa kokukhula, okuhlinzeka ngedatha yokubhekisela ekusetshenzisweni kabanzi kwalezi zinto ezimbili ezintsha ezikhangayo embonini yokudla kanye neyokufuya izilwane zasemanzini.

1 Izinto Nezindlela

1.1 Izinto zokuhlola kanye nezinhlanzi zokuhlola
I-S. S' - I-Dimethylacetic acid thiazole (I-DMT), i-DMPT
Izinhlanzi zokuhlola zithathwe epulazini lokufuya izilwane zasemanzini, ezinemizimba enempilo kanye nemininingwane ecacile. Ngaphambi kokuba kuqale ukuhlolwa ngokusemthethweni, izinhlanzi zokuhlola zizokhuliswa okwesikhashana elabhorethri izinsuku eziyisikhombisa, lapho zizokondliwa khona ngokudla kwezinhlanzi ze-carp okuhlinzekwa yifektri yokudla.
1.2 Okuphakelayo kokuhlola
1.2.1 Ukudla kokuhlola ukuthaka: Cindezela ukudla kwe-carp okuhlinzekwa yifektri yokudla, engeza inani elilinganayo lesitashi esingu-A, hlanganisa ngokulinganayo, bese uxuba nenani elifanele lamanzi acwengekile ukuze wenze amabhola anamathelayo angu-5g ngalinye njengokudla kweqembu lokulawula. Ngesikhathi esifanayo, lungisa ukudla kwe-bait ngokuchoboza ukudla kwe-carp kuqala, wengeze inani elilinganayo lesitashi esingu-alpha, bese ufaka i-bait DMT kanyeI-DMPTngamanani amabili angu-0.5g/kg kanye no-1g/kg, ngokulandelana. Hlanganisa ngokulinganayo bese uxuba nenani elifanele lamanzi acwengekile ukuze wenze ibhola ngalinye elinamathelayo elingu-5g.
1.2.2 Ukudla kokuhlola ukukhula:

Sika ukudla kwe-carp (okuvela emthonjeni ofanayo nongenhla) kube yimpuphu, kudlulise ngesihlungo sama-mesh angu-60, engeza inani elilinganayo lesitashi se-alpha, uhlanganise kahle, uhlanganise namanzi acwengekile, ukucindezele kusuka kusihlungo kube ama-granule, bese ukomisa emoyeni ukuze uthole ukudla kweqembu lokulawula lokuhlolwa kokukhula.I-DMTkanye namakristalu e-DMPT ancibilikiswa emanzini acwengekile ukuze kulungiselelwe isixazululo sokuhlushwa okufanele, esasetshenziswa ukuxuba ukudla kwe-carp okuxutshwe kahle kanye nesitashi kube ama-granule. Ngemva kokomiswa, ukudla kweqembu lokuhlola kwatholakala, kanyeI-DMTkanye ne-DMPT yengezwe ngezigaba ezintathu zokuhlushwa okungu-0.1g/kg, 0.2g/kg, kanye no-0.3g/kg, ngokulandelana.

1.3 Indlela Yokuhlola
1.3.1 Ukuhlolwa kwe-Lure: Khetha izinhlanzi ezi-5 ezihlolwayo (ezinesisindo esimaphakathi esingu-30g) njengezinhlanzi ezihlolwayo. Ngaphambi kokuhlolwa, yilambe amahora angama-24, bese ubeka inhlanzi ehlolwayo e-aquarium eyingilazi (enosayizi ongu-40 × 30 × 25cm). Ukudla kwe-lure kuqiniswe ebangeni elingama-5.0cm ukusuka phansi kwe-aquarium usebenzisa umugqa olengisiwe oboshwe kubha evundlile. Inhlanzi iluma i-lure bese idlidliza umugqa, odluliselwa kubha evundlile bese uqoshwa yi-wheel recorder. Imvamisa yokuluma i-lure ibalwa ngokusekelwe ekudlidlizeni okuphezulu kwezinhlanzi ezi-5 ezihlolwayo eziluma i-lure zingakapheli imizuzu emi-2. Ukuhlolwa kokudla kweqembu ngalinye lokudla kwaphindwa kathathu, kusetshenziswa amabhola okunamathela okudla asanda kulungiswa isikhathi ngasinye. Ngokwenza ukuhlolwa okuphindaphindiwe ukuthola inani eliphelele kanye nemvamisa evamile yokudlidliza, umphumela wokudla weI-DMTkanye ne-DMPT ku-carp kungahlolwa.

1.3.2 Ukuhlolwa kokukhula kusebenzisa ama-aquarium ayingilazi ayi-8 (usayizi ongu-55 × 45 × 50cm), anokujula kwamanzi okungu-40cm, izinga lokushisa lamanzi lemvelo, kanye nokwehla okuqhubekayo. Izinhlanzi zokuhlola zabelwa ngokungahleliwe futhi zahlukaniswa ngamaqembu amabili okuhlolwa. Iqembu lokuqala liqukethe ama-aquarium amane, anenombolo engu-X1 (iqembu lokulawula), i-X2 (ukudla okungu-0.1gDMT/kg), i-X3 (ukudla okungu-0.2gDMT/kg), i-X4 (ukudla okungu-0.3gDMT/kg); Elinye iqembu lama-aquarium amane, anenombolo engu-Y1 (iqembu lokulawula), i-Y2 (ukudla okungu-0.10g DMPT/kg), i-Y3 (ukudla okungu-0.2g DMPT/kg), i-Y4 (ukudla okungu-0.30g DMPT/kg). Izinhlanzi ezingu-20 ebhokisini ngalinye, ziphakelwa izikhathi ezintathu ngosuku ngo-8:00, 13:00, kanye no-17:00, ngesilinganiso sokudla sansuku zonke esingu-5-7% wesisindo somzimba. Ukuhlolwa kwathatha amasonto ayi-6. Ekuqaleni nasekupheleni kokuhlolwa, kwalinganiswa isisindo esimanzi senhlanzi yokuhlola futhi kwaqoshwa izinga lokusinda kweqembu ngalinye.

2.1 Umphumela wokudla we-DMPT kanyeI-DMTku-carp
Umphumela wokudla we-DMPT kanyeI-DMTku-carp kubonakala ngokuvama kokuluma kwezinhlanzi zokuhlola ngesikhathi sokuhlolwa kwemizuzu emi-2, njengoba kuboniswe kuThebula 1. Ukuhlolwa kuthole ukuthi ngemva kokufaka ukudla kwe-DMPT kanye ne-DMT ku-aquarium, izinhlanzi zokuhlola zasheshe zabonisa ukuziphatha okusebenzayo kokufuna ukudla, kuyilapho lapho kusetshenziswa ukudla kweqembu lokulawula, ukusabela kwezinhlanzi zokuhlola kwakuhamba kancane. Uma kuqhathaniswa nokudla kokulawula, izinhlanzi zokuhlola zanda kakhulu ngokuvama kokuluma ukudla kokuhlola. I-DMT kanye ne-DMPT zinemiphumela ekhangayo ekhanga kakhulu ku-carp yokuhlola.

Izinga lokukhuluphala kwesisindo, izinga lokukhula elithile, kanye nezinga lokusinda kwe-carp ephakelwa ngamanani ahlukene e-DMPT kwandiswe kakhulu uma kuqhathaniswa nalawo aphakelwa ngokudla okulawulayo, kuyilapho i-coefficient yokudla yehlisiwe kakhulu. Phakathi kwawo, ukungezwa kwe-DMPT ku-T2, T3, kanye ne-T4 kwandise ukukhuluphala kwesisindo kwansuku zonke kwamaqembu amathathu ngo-52.94%, 78.43%, kanye no-113.73%, ngokulandelana, uma kuqhathaniswa neqembu lokulawula. Izinga lokukhuluphala kwesisindo le-T2, T3, kanye ne-T4 landa ngo-60.44%, 73.85%, kanye no-98.49%, ngokulandelana, kanti amazinga okukhula athile anda ngo-41.22%, 51.15%, kanye no-60.31%, ngokulandelana. Izinga lokusinda landa kusuka ku-90% kuya ku-95%, kanti ama-coefficient okuphakelayo anciphe ngo-28.01%, 29.41%, kanye no-33.05%, ngokulandelana.

3. Isiphetho

Kulolu vivinyo, ngabeI-DMTnoma i-DMPT yengezwe, imvamisa yokudla, izinga lokukhula elithile, kanye nokukhuphuka kwesisindo kwansuku zonke kwezinhlanzi zokuhlola eqenjini ngalinye kwanda kakhulu uma kuqhathaniswa neqembu lokulawula, kuyilapho i-coefficient yokudla yehla kakhulu. Futhi kungakhathaliseki ukuthi yi-DMT noma i-DMPT, umphumela wokukhuthaza ukukhula uba mkhulu kakhulu ngokwanda kwenani lokwengeza kumazinga amathathu angu-0.1g/kg, 0.2g/kg, kanye no-0.3g/kg. Ngesikhathi esifanayo, kwenziwa ukuqhathaniswa kwemiphumela yokondla nokukhuthaza ukukhula kwe-DMT ne-DMPT. Kwatholakala ukuthi ngaphansi kokuhlushwa okufanayo kokugunda izinwele, imvamisa yokondla, izinga lokuzuza isisindo, kanye nezinga lokukhula elithile lezinhlanzi zokuhlola eqenjini lokudla le-DMPT kwanda kakhulu uma kuqhathaniswa neqembu lokudla le-DMT, kuyilapho i-coefficient yokondla yehliswe kakhulu. Uma sikhuluma ngokulinganiselwe, i-DMPT inomphumela obaluleke kakhulu ekudonseni nasekukhuthazeni ukukhula kwe-carp uma kuqhathaniswa ne-DMT. Lokhu kuhlola kusebenzise i-DMPT ne-DMT engezwe ekudleni kwe-carp ukuze kuhlolwe imiphumela yazo yokondla nokukhuthaza ukukhula. Imiphumela ikhombisa ukuthi i-DMPT ne-DMT zinamathemba okusetshenziswa kabanzi njengesizukulwane esisha sezilwane zasemanzini ezikhangayo.