Ukuze kuhlolwe umphumela wokufaka i-triglyceride ekudleni ekukhiqizweni kwamaprotheni amagciwane e-rumen kanye nezici zokubilisa zezimvukazi ezincane ezindala ezinomsila, kwenziwa izivivinyo ezimbili ku-vitro kanye naku-vivo.

Ukuhlolwa kwe-in vitro: ukudla okuyisisekelo (okusekelwe ezintweni ezomile) okunezinga le-triglyceride elingu-0, 2, 4, 6 kanye no-8g / kg kwasetshenziswa njengesisekelo, kwathi ijusi le-rumen lezimvukazi ze-Adult Small Tailed lanezelwa, labe selifakwa esikhumbeni ku-39 ℃ amahora angu-48 kwe-in vitro.

Ukuhlolwa kwe-in vivo: izimvukazi ezindala ezingu-45 zahlukaniswa ngokungahleliwe zaba amaqembu ama-5 ngokwesisindo sazo sokuqala (55 ± 5 kg).i-glyceryl tributylatekwengezwe u-0, 2, 4, 6 kanye no-8 g/kg (ngokusekelwe ezintweni ezomile) ekudleni okuyisisekelo, futhi uketshezi lwesisu kanye nomchamo kwaqoqwa izinsuku ezingu-18.

Umphumela Wokuhlola

1). Umphumela enanini le-pH kanye nokuhlushwa kwe-fatty acid eguquguqukayo

Imiphumela ikhombisile ukuthi inani le-pH le-culture medium lehle ngokulandelana kanti ukugcwala kwama-total volatile fatty acids (TVFA), i-acetic acid, i-butyric acid kanye nama-branched chain volatile fatty acids (BCVFA) kwanda ngokulandelana laphoi-tributyl glyceridekwengezwe ku-substrate. Imiphumela yokuhlolwa kwe-in vivo ibonise ukuthi inani le-dry matter intake (DMI) kanye ne-pH lehlile, kanti ukugcwala kwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kanye ne-BCVFA kwanda ngokulandelana ngokufakwa kwe-i-tributyl glycerideImiphumela yokuhlolwa kwe-in vivo ibonise ukuthi inani le-dry matter intake (DMI) kanye ne-pH lehlile, kanti ukugcwala kwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kanye ne-BCVFA kwanda ngokulandelana ngokufakwa kwe-tributyl glyceride.

Imiphumela yokuhlolwa kwe-in vivo ibonise ukuthi inani le-dry matter intake (DMI) kanye ne-pH lehlile, kanti ukugcwala kwe-TVFA, i-acetic acid, i-propionic acid, i-butyric acid kanye ne-BCVFA kwanda ngokulandelana ngokufakwa kwe-i-tributyl glyceride.

2). Thuthukisa izinga lokuwohloka kwezakhamzimba

Izinga lokuwohloka okubonakalayo kwe-DM, CP, NDF kanye ne-ADF lenyuke ngokulandelana laphoi-tributyl glyceridekwanezelwa ku-substrate in vitro.

3). Thuthukisa umsebenzi we-enzyme ebhubhisa i-cellulose

Ukwengezwa kwei-tributyrinUkuhlolwa kwe-in vitro kwandisa imisebenzi ye-xylanase, i-carboxymethyl cellulase kanye ne-microcrystalline cellulase. Ukuhlolwa kwe-in vivo kubonise ukuthi i-triglyceride kwandisa imisebenzi ye-xylanase kanye ne-carboxymethyl cellulase.

4). Thuthukisa ukukhiqizwa kwamaprotheni amagciwane

Ukuhlolwa kwe-in vivo kubonise ukuthii-tributyrinkwandise ngokulinganayo inani lansuku zonke le-allantoin, i-uric acid kanye ne-purine emuncwa yi-microbial emchameni, futhi kwandise ukwakheka kwe-rumen microbial nitrogen.

Isiphetho

I-Tributyrinkuthuthukise ukwenziwa kweprotheyini yamagciwane e-rumen, okuqukethwe kwama-fatty acid aphelele aguquguqukayo kanye nomsebenzi wama-enzyme abhubhisa i-cellulose, futhi kwakhuthaza ukuwohloka nokusetshenziswa kwezinto ezomile, iphrotheyini eluhlaza, i-neutral detergent fiber kanye ne-acid detergent fiber ekudleni.

Kubonisa ukuthi i-triglyceride inomthelela omuhle ekuvunweni nasekuvuthweni kweprotheyini yamagciwane e-rumen, futhi ingaba nomthelela omuhle ekusebenzeni kahle kokukhiqizwa kwezimvu ezindala.