I-Guanylacetic acid, eyaziwa nangokuthi i-guanylacetic acid, iyi-analogue ye-amino acid eyakhiwe nge-glycine ne-L-lysine.

I-Guanylacetic acid ingahlanganisa i-creatine ngaphansi kwe-catalysis yama-enzyme futhi iyona kuphela imfuneko yokwenziwa kwe-creatine. I-Creatine iqashelwa njenge-energy buffer, futhi umsebenzi wayo oyinhloko ukwakha i-creatine e-phosphorylated ngaphansi kwesenzo se-creatine kinase.

Bamba iqhaza ohambweni lwe-adenosineumjikelezo we-hosphate (ATP). Uma amandla e-ATP enganele, i-phosphocreatine idlulisela ngokushesha iqembu le-phosphate ku-adenosine diphosphate nge-creatine kinase bese iliguqula libe yi-adenosine triphosphate.

 

Ukusetshenziswa kwezilwane ezincelisayo:

Ukwengeza i-guanylacetic acid engu-0.12%, 0.08%, kanye no-0.04% ekudleni kwezimvu ezingu-120 ezifuywe ngebhari ezinesisindo esingamakhilogremu angu-20, ngokulandelana, kubonise ukuthi ukwengezwa kwe-guanylacetic acid engu-0.12% kanye no-0.08% kwandisa kakhulu ukukhuluphala kwansuku zonke, amafutha emisipha, kanye nokuqukethwe kwamaprotheni, kanye nokwehla okukhulu kwamafutha esidumbu.

 

Ukwengezwa kuka-0.08%i-guanylacetic acidkwanda iphesenti lenyama elihlanzekile ngo-9.77%. Ngokusebenzisa indlela yokukhiqiza igesi ye-in vitro, kwafundwa umphumela wokwengeza amazinga ahlukene e-guanylacetic acid ku-rumen yezinkomo eziphuzi. Kwatholakala ukuthi ukungezwa kwe-guanylacetic acid engu-0.4% kwandise kakhulu ukukhiqizwa kwegesi, kanti ukuhlushwa kwe-ammonia nitrogen kuqala kwanda kwabe sekwehla.

Ngakho-ke, kungaphethwa ngokuthi ukwengeza i-guanylacetic acid ekudleni kwansuku zonke kungathuthukisa indawo yangaphakathi ye-rumen kanye nendlela yokubilisa izinkomo eziphuzi.

Ukusetshenziswa kwezinkukhu:

Ukwengeza i-800 mg/kg, i-1600 mg/kg, i-4000 mg/kg, kanye ne-8000 mg/kg ye-guanylacetic acid ekudleni kwansuku zonke kwezinkukhu kubonise ukuthi ukufaka i-800-4000 mg/kg ye-guanylacetic acid ekudleni kwandise kakhulu inzuzo yesisindo yansuku zonke yezinkukhu, kunciphisa isilinganiso sokudla nesisindo sezinkukhu ezinsukwini ezingu-22-42 ubudala. Ukwengeza i-8000 mg/kg ye-guanylacetic acid kuthuthukise izinkomba ze-biochemical ze-serum njenge-urea nitrogen, izinkomba zesimiso segazi, kanye ne-bilirubin iyonke Akukho mthelela omkhulu ezinkomba zezitho ezinkulu, okubonisa ukuthi ukufaka i-8000 mg/kg ye-guanylacetic acid ekudleni kwansuku zonke kwezinkukhu kuyabekezeleleka.

Ukwengeza u-200 mg/kg, u-400 mg/kg, u-600 mg/kg, kanye no-800 mg/kg we-guanylacetic acid ekudleni kwenkukhu kubonise ukwanda okukhulu kokukhuluphala kwesisindo kwansuku zonke uma kuqhathaniswa neqembu lokulawula. Imiphumela emihle kakhulu yatholakala lapho amazinga okwengeza ayengu-600 no-800 mg/kg.

Ukuze kufundwe umphumela we-guanylacetic acid ekhwalithini yesidoda emaqhudeni, kukhethwe amaqhude angu-20 anamasonto angu-28 ukuze ondle ukudla okuqukethe i-guanylacetic acid engu-0%, 0.06%, 0.12%, kanye ne-0.18% ye-guanylacetic acid. Imiphumela yocwaningo ithole ukuthi ukwengeza i-guanylacetic acid engu-0.12% ekudleni kwandisa kakhulu inani lesidoda, ukuhlushwa kwesidoda, kanye nomsebenzi wesidoda emaqhudeni, okubonisa ukuthi ukwengeza i-guanylacetic acid ekudleni kungathuthukisa ngempumelelo ikhwalithi yesidoda. Engeza i-guanylacetic acid engu-0.0314%, 0.0628%, 0.0942%, kanye ne-0.1256% ekudleni kwansuku zonke kwama-broiler, bese ubeka amaqembu amabili okulawula (iqembu lokulawula 1 ukudla okusekelwe ezitshalweni ngaphandle kwento engeziwe, kanti iqembu lokulawula 2 ukudla okunesidlo senhlanzi esingeziwe). Amaqembu ayisithupha angenhla okudla kwansuku zonke anezinga elifanayo lamandla namaminerali.

Imiphumela yokuhlola ikhombisa ukuthi amazinga okuzuza isisindo samaqembu amane ane-guanylacetic acid eyengeziwe kanye neqembu lokulawula 2 aphakeme kunalawo eqembu lokulawula 1, Iqembu lokulawula 2 libe nomphumela omuhle kakhulu wokuzuza isisindo, kulandelwa iqembu le-guanylacetic acid elingu-0.0942%; Iqembu lokulawula 2 libe nesilinganiso esihle kakhulu sezinto ezibonakalayo nesisindo, kulandelwe iqembu le-guanylacetic acid elingu-0.1256%.

Ukusetshenziswa kwezinkukhu:

Ukwengeza u-800 mg/kg, 1600 mg/kg, 4000 mg/kg, kanye no-8000 mg/kg wei-guanylacetic acidekudleni kwansuku zonke kwenkukhu ezifuywayo kubonise ukuthi ukufaka i-800-4000 mg/kg ye-guanylacetic acid ekudleni kwandise kakhulu ukukhuphuka kwesisindo kwansuku zonke kwenkukhu ezifuywayo, kwehlisa isilinganiso sokudla kuya kwesisindo senkukhu ezifuywayo ezinsukwini ezingama-22-42 ubudala. Ukwengeza i-8000 mg/kg ye-guanylacetic acid ethuthukisiwe izinkomba ze-biochemical serum ezifana ne-urea nitrogen, izinkomba zesimiso segazi, kanye ne-bilirubin iyonke Akukho mthelela omkhulu ezinkombeni ezinkulu zezitho, okubonisa ukuthi ukufaka i-8000 mg/kg ye-guanylacetic acid ekudleni kwansuku zonke kwenkukhu ezifuywayo kuyabekezeleleka. Ukwengeza i-200 mg/kg, 400 mg/kg, 600 mg/kg, kanye ne-800 mg/kg ye-guanylacetic acid ekudleni kwenkukhu ezifuywayo kubonise ukwanda okukhulu kwesilinganiso sokwanda kwesisindo kwansuku zonke uma kuqhathaniswa neqembu lokulawula. Imiphumela emihle kakhulu yatholakala lapho amazinga okwengeza ayengu-600 no-800 mg/kg.

Ukuze kufundwe umphumela we-guanylacetic acid ekhwalithini yesidoda emaqhudeni, kukhethwe amaqhude angu-20 anamasonto angu-28 ukuze ondle ukudla okuqukethe i-guanylacetic acid engu-0%, 0.06%, 0.12%, kanye ne-0.18% ye-guanylacetic acid. Imiphumela yocwaningo ithole ukuthi ukwengeza i-guanylacetic acid engu-0.12% ekudleni kwandisa kakhulu inani lesidoda, ukuhlushwa kwesidoda, kanye nomsebenzi wesidoda emaqhudeni, okubonisa ukuthi ukwengeza i-guanylacetic acid ekudleni kungathuthukisa ngempumelelo ikhwalithi yesidoda. Engeza i-guanylacetic acid engu-0.0314%, 0.0628%, 0.0942%, kanye ne-0.1256% ekudleni kwansuku zonke kwama-broiler, bese ubeka amaqembu amabili okulawula (iqembu lokulawula 1 ukudla okusekelwe ezitshalweni ngaphandle kwento engeziwe, kanti iqembu lokulawula 2 ukudla okunesidlo senhlanzi esingeziwe). Amaqembu ayisithupha angenhla okudla kwansuku zonke anezinga elifanayo lamandla namaminerali. Imiphumela yokuhlola ikhombisa ukuthi amazinga okuzuza isisindo samaqembu amane ane-guanylacetic acid eyengeziwe kanye neqembu lokulawula 2 aphakeme kunalawo eqembu lokulawula 1, Iqembu lokulawula 2 libe nomphumela omuhle kakhulu wokuzuza isisindo, kulandelwa yi-0.0942%i-guanylacetic acidiqembu lokulawula 2 lalinokulinganisela okungcono kakhulu kwezinto ezibonakalayo nesisindo, kulandelwa iqembu le-guanylacetic acid elingu-0.1256%.