Ukufakwa esikhundleni kwengxenye yenyama yenhlanzi ngenyama yesoya (i-SBM) njengendlela eqhubekayo neyomnotho kuye kwahlolwa ezinhlotsheni eziningi zezinhlanzi ezihlosiwe kwezentengiselwano, okuhlanganisa nezinhlanzi zasolwandle ezinamanzi ahlanzekile (i-Freshwater rainbow trout) (I-Oncorhynchus mykiss). Kodwa-ke, i-soy nezinye izinto ezisekelwe ezitshalweni ziqukethe amazinga aphezulu ama-saponins nezinye izinto ezilwa nokudla okunempilo ezibangela i-subacute enteritis yamathumbu akude kulezi zinhlanzi eziningi. Lesi simo sibonakala ngokwanda kokungena kwamathumbu, ukuvuvukala, kanye nokukhubazeka kokwakheka okuholela ekunciphiseni ukusebenza kahle kokudla kanye nokukhula okuphazamisekile.

Ku-rainbow trout, kufaka phakathi i-SBM ngaphezu kwama-20% okudla kuye kwabonakala ukuthi kubangela i-soy-enteritis, okubeka umkhawulo womzimba ezingeni elingashintshwa ekudleni okujwayelekile kwe-aquaculture. Ucwaningo lwangaphambilini luhlole izindlela eziningana zokulwa nalesi sifo sokuwa, okuhlanganisa ukuphathwa kwe-microbiome yamathumbu, ukucubungula izithako ukuze kususwe izinto ezilwa nokudla okunempilo, kanye nezithasiselo ze-antioxidant kanye ne-probiotic. Enye indlela engakahlolwa ukufaka i-trimethylamine oxide (TMAO) ekudleni kwe-aquaculture. I-TMAO iyi-cytoprotective yendawo yonke, eqoqwe ezinhlotsheni eziningi njenge-protein kanye ne-membrane stabilizer. Lapha, sihlola ikhono le-TMAO lokuthuthukisa ukuzinza kwe-enterocyte nokucindezela isignali ye-HSP70 yokuvuvukala ngaleyo ndlela silwe ne-enteritis ebangelwa yi-soy futhi kuholele ekwandeni kokusebenza kahle kokudla, ukugcinwa kanye nokukhula kwe-rainbow trout yamanzi ahlanzekile. Ngaphezu kwalokho, sihlola ukuthi izinhlayiya zezinhlanzi zasolwandle, umthombo ocebile we-TMAO, zingasetshenziswa njengendlela ewusizo kwezomnotho yokuphatha lesi sithasiselo, okwenza ukusetshenziswa kwaso kube lula ebhizinisini.

I-trout efuywayo (i-Troutlodge Inc.) yayigcwele isisindo sokuqala esingu-40 g kanye no-n=15 ngethangi ngalinye ematangini okwelapha aphindwe kathathu. Amatangi anikwa ukudla okukodwa kweziyisithupha okulungiselelwe ngesisekelo sezakhamzimba ezigaywayo ezinikeza amaprotheni agaywayo angu-40%, amafutha aluhlaza angu-15%, futhi ahlangabezana nokuhlushwa kwama-amino acid afanele. Ukudla kwakuhlanganisa ukudla kwenhlanzi okungu-40 okulawulayo (% yokudla okomile), i-SBM 40, i-SBM 40 + i-TMAO engu-3 g kg.^-1, SBM 40 + TMAO 10 g kg^-1, SBM 40 + TMAO 30 g kg^-1, kanye ne-SBM 40 + 10% yezinto ezincibilikayo ezinhlanzini. Amathangi anikwa ukudla kabili ngosuku ukuze asuthe amasonto ayi-12 futhi kwenziwa ukuhlaziywa kwendle, eduze, kwe-histological kanye ne-molecular.

Imiphumela yalolu cwaningo izoxoxwa ngayo kanye nokubaluleka kokufaka i-TMAO ukuze kuvunyelwe ukusetshenziswa okuphezulu kwemikhiqizo yesoya yase-US ekudleni kwasolwandle kwe-salmonid.