AbstractUcwaningo lwenziwa ukutadisha imiphumela ye-diludine ekubekeni ukusebenza kanye nekhwalithi yeqanda ezikhukhukazini kanye nendlela yokwenza imiphumela ngokunquma izinkomba zeqanda nemingcele ye-serum 1024 ROM izikhukhukazi zahlukaniswa ngamaqembu amane ngalinye elihlanganisa ukuphindaphinda okune kwezinkukhu ezingu-64 ngayinye, Amaqembu okwelapha athola ukudla okufanayo kwe-basal okune-0, 2000000000000 mg, i-2010000000000 mg, i-dilue, i-2, i-10000000000000/0, i-10000000 mg / kg. 80d. Imiphumela yaba kanje. Ukwengezwa kwe-diludine ekudleni kwathuthukisa ukusebenza kwezinkukhu ezizalelayo, lapho ukwelashwa okungu-150 mg/kg kwakungcono kakhulu; izinga layo lokulala lenyuke ngo-11.8% (p< 0.01), ukuguqulwa kwenqwaba yamaqanda kwehle ngo-10.36% (p< 0 01). Izisindo zamaqanda zanda ngokukhula kwe-diludine yengezwe. I-Diludine yehlise kakhulu ukugcwala kwe-uric acid ku-serum (p<0.01); ukwengeza i-diludine kwehlisa kakhulu i-serum Ca²⁺kanye nokuqukethwe kwe-phosphate ye-inorganic, kanye nomsebenzi owandisiwe we-alkine phosphatase (ALP) ye-serum (p<0.05), ngakho kwaba nemiphumela ebalulekile ekwehliseni ukuphuka kwamaqanda (p<0.05) nokungajwayelekile (p <0.05); i-diludine yandisa kakhulu ukuphakama kwe-albhamuen. Inani le-Haugh (p <0.01), ukujiya kwegobolondo kanye nesisindo segobolondo (p< 0.05), 150 kanye no-200mg/kg i-diludine nayo yehlise isamba se-choleasterol ku-yolk yeqanda (p< 0 05), kodwa yanda isisindo se-yolk yeqanda (p <0.05). Ngaphezu kwalokho, i-diludine ingathuthukisa umsebenzi we-lipase (p <0.01), futhi yehlise okuqukethwe kwe-triglyceride (TG3) (p<0.01) ne-cholesterol (CHL) (p<0 01) ku-serum, yehlisa iphesenti lamafutha esisu (p<0.01) kanye nokuqukethwe kwamafutha esibindi (p

Amagama ayisihluthuleloI-Diludine; isikhukhukazi; I-SOD; i-cholesterol; triglycerides, lipase

 

I-diludine iyinoveli engeyona i-nutritive anti-oxidation vitamin additive futhi inemiphumela^[1-3]yokuvimbela i-oxidation yolwelwesi lwebhayoloji kanye nokuzinzisa izicubu zamangqamuzana ezinto eziphilayo, njll. Ngawo-1970, uchwepheshe wezolimo waseLatvia ezweni elaliyiSoviet Union wathola ukuthi i-diludine yayinemiphumela.^[4]wokuthuthukisa ukukhula kwezinkukhu kanye nokumelana nokubanda nokuguga kwezinye izitshalo. Kwabikwa ukuthi i-diludine ayigcini nje ngokukhuthaza ukukhula kwesilwane, kodwa ithuthukise ukusebenza kokuzala kwesilwane ngokusobala futhi ithuthukise izinga lokukhulelwa, ukuphuma kobisi, ukuphuma kweqanda kanye nezinga lokuchanyuselwa kwesilwane sesifazane.^{[1, 2, 5-7]}. Ucwaningo lwe-diludine e-china lwaqalwa kusukela ngawo-1980, futhi iningi lezifundo mayelana ne-diludine eShayina lisebenzisa umphumela kuze kube manje, futhi izivivinyo ezimbalwa zokubekela izinyoni zabikwa. U-Chen Jufang (1993) ubike ukuthi i-diludine ingathuthukisa ukuphuma kweqanda nesisindo seqanda lezinkukhu, kodwa ayizange ijule.^[5]isifundo sendlela yokusebenza kwayo. Ngakho-ke, senze ucwaningo oluhlelekile lomphumela nendlela yakho ngokuphakela izinkukhu ezizalelayo ngokudla okufakwe i-diludine, futhi ingxenye eyodwa yomphumela manje ibikwa kanje:

Ithebula 1 Ukwakheka kanye nezingxenye zemisoco yokudla okuhlolwayo

%

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Ukwakheka kokudla Izakhi zomsoco

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Ummbila 62 ME③ 11.97

I-pulp kabhontshisi 20 CP 17.8

Isidlo sezinhlanzi 3 Ca 3.42

Isidlo sokudlwengulwa 5 P 0.75

Ukudla kwamathambo 2 M et 0.43

Ukudla kwamatshe 7.5 M et Cys 0.75

I-Methionine 0.1

Usawoti 0.3

I-Multivitamin① 10

Landela izakhi② 0.1

---------------------------------------------------------------------------------------

① I-Multivitamin: 11mg ye-riboflavin, 26mg ye-folic acid, 44mg ye-oryzanin, 66mg ye-niacin, 0.22mg ye-biotin, 66mg ye-B6, 17.6ug ye-B12, 880mg ye-choline, 30mg ye-VK, 66IU_E, 6600ICU ye-V_Dkanye ne-20000ICU ye-V_A, zengezwe kilogram ngayinye yokudla; kanye ne-10g ye-multivitamin yengezwe ku-50kg ngayinye yokudla.

② Izakhi zokulandelela (mg/kg): 60 mg we-Mn, 60mg we-Zn, 80mg we-Fe, 10mg we-Cu, 0.35mg we-I kanye no-0.3mg we-Se wengezwa kukhilogremu ngayinye yokudla.

③ Iyunithi yamandla agaywayo isho i-MJ/kg.

 

1. Izinto kanye nendlela

1.1 Izinto zokuhlola

I-Beijing Sunpu Biochem. & Ubuchwepheshe. I-Co., Ltd. kufanele inikeze i-diludine; futhi isilwane esivivinyayo sizobhekisela ezinkukhu zamazalela zaseRoma ezinezinsuku ezingama-300 ubudala.

 

Ukudla okuhlolwayo: ukudla okuhlolwayo kufanele kulungiswe ngokuya ngesimo sangempela ngesikhathi sokukhiqiza ngesisekelo sezinga le-NRC, njengoba kukhonjisiwe kuThebula 1.

1.2 Indlela yokuhlola

1.2.1 Ukuhlolwa kokuphakelayo: ukuhlolwa kokuphakela kufanele kwenziwe epulazini leNkampani yaseHongji eDolobheni laseJiande; 1024 Izikhukhukazi zamaRoma ezizalelayo kufanele zikhethwe futhi zihlukaniswe ngamaqembu amane ngokungahleliwe futhi ngalinye libe yizicucu ezingu-256 (iqembu ngalinye kufanele liphindwe izikhathi ezine, futhi inkukhu ngayinye kufanele iphindwe izikhathi ezingu-64); izikhukhukazi kufanele zidliwe ngezidlo ezine ezinokuqukethwe okuhlukene kwe-diludine, futhi u-0, 100, 150, 200mg/kg wokudla kufanele kwengezwe eqenjini ngalinye. Ukuhlolwa kwaqalwa ngo-April 10, 1997; nezikhukhukazi zazingathola ukudla futhi zithathe amanzi mahhala. Ukudla okuthathwe yiqembu ngalinye, izinga lokuzala, ukuphuma kweqanda, iqanda eliphukile kanye nenani leqanda elingavamile kufanele kurekhodwe. Ngaphezu kwalokho, ukuhlolwa kwaqedwa ngo-June 30, 1997.

1.2.2 Ukulinganisa ikhwalithi yamaqanda: Amaqanda angu-20 kufanele athathwe ngokungahleliwe lapho ukuhlolwa kwenziwa izinsuku ezine ezingu-40 ukuze kulinganiswe izinkomba ezihlobene nekhwalithi yeqanda, njengenkomba yokwakheka kweqanda, iyunithi ye-haugh, isisindo esilinganiselwe segobolondo, ubukhulu begobolondo, inkomba ye-yolk, isisindo esilinganiselwe se-yolk, njll. Ngaphezu kwalokho, okuqukethwe kwe-cholesterol ku-yolk indlela ye-COD reagent kufanele kukhiqizwe nge-cod reagent ye-COD I-Ningbo Cixi Biochemical Test Plant.

1.2.3 Ukukalwa kwe-serum biochemical index: Izikhukhukazi zokuhlola eziyi-16 kufanele zithathwe eqenjini ngalinye isikhathi ngasinye lapho ukuhlolwa kwenziwa izinsuku ezingama-30 nalapho ukuhlolwa sekuqediwe ukuze kulungiswe i-serum ngemva kokuthatha isampula yegazi emthanjeni ophikweni. I-serum kufanele igcinwe ezingeni lokushisa eliphansi (-20 ℃) ​​ukuze kulinganiswe izinkomba ezifanele ze-biochemical. Iphesenti lamafutha esisu kanye ne-lipid yesibindi kufanele kulinganiswe ngemva kokuhlatshwa nokukhipha amanoni esisu kanye nesibindi lapho kuqedwa ukuthatha amasampula egazi.

I-superoxide dismutase (SOD) kufanele ikalwe kusetshenziswa indlela yokugcwala kwamanzi phambi kwekhithi ye-reagent ekhiqizwe yi-Beijing Huaqing Biochem. & Ubuchwepheshe. Isikhungo Socwaningo. I-uric acid (UN) ku-serum kufanele ikalwe ngokusebenzisa indlela ye-U ricase-PAP phambi kwekhithi ye-Cicheng reagent; i-triglyceride (TG3) kufanele ikalwe ngokusebenzisa indlela yesinyathelo esisodwa ye-GPO-PAP phambi kwekhithi ye-Cicheng reagent; i-lipase kufanele ilinganiswe ngokusebenzisa i-nephelometry phambi kwekhithi ye-Cicheng reagent; i-serum total cholesterol (CHL) kufanele ikalwe ngokusebenzisa indlela ye-COD-PAP phambi kwekhithi ye-Cicheng reagent; i-glutamic-pyruvic transaminase (GPT) kufanele ikalwe ngokusebenzisa i-colorimetry phambi kwekhithi ye-Cicheng reagent; i-glutamic-oxalacetic transaminase (GOT) kufanele ikalwe ngokusebenzisa i-colorimetry phambi kwekhithi ye-Cicheng reagent; i-alkaline phosphatase (ALP) kufanele ikalwe ngokusebenzisa indlela yesilinganiso phambi kwekhithi ye-Cicheng reagent; i-calcium ion (Ca²⁺) ku-serum kufanele kulinganiswe ngokusebenzisa indlela ye-methylthymol blue complexone phambi kwekhithi ye-Cicheng reagent; i-phosphorus ye-inorganic (P) kufanele ikalwe ngokusebenzisa indlela eluhlaza okwesibhakabhaka ye-molybdate phambi kwekhithi ye-Cicheng reagent.

 

2 Umphumela wokuhlolwa

2.1 Umthelela ekusebenzeni kokubeka

Imisebenzi yokubeka yamaqembu ahlukene asetshenzwe kusetshenziswa i-diludine ikhonjiswe kuThebula lesi-2.

Ithebula 2 Ukusebenza kwezikhukhukazi ezidliwe nge-basal diet ehambisana namazinga amane e-diludine

 

Inani le-diludine elizongezwa (mg/kg)
	0	100	150	200
Ukudla okuphakelayo (g)
Izinga lokubeka (%)
Isisindo esimaphakathi seqanda (g)
Isilinganiso sento neqanda
Izinga leqanda eliphukile (%)
Izinga leqanda elingavamile (%)

 

Kusukela kuThebula lesi-2, amazinga okubekwa kwawo wonke amaqembu acutshungulwa ngokusebenzisa i-diludine athuthukiswa ngokusobala, lapho umphumela uma ucutshungulwa ngokusebenzisa i-150mg/kg ulungile (kufika ku-83.36%), futhi i-11.03% (p<0.01) ithuthukisiwe uma iqhathaniswa neqembu lereferensi; ngakho-ke i-diludine inomphumela wokuthuthukisa izinga lokubeka. Uma kubhekwa isisindo esijwayelekile seqanda, isisindo seqanda siyakhula (p>0.05) kanye nokwanda kwe-diludine ekudleni kwansuku zonke. Uma kuqhathaniswa neqembu lereferensi, umehluko phakathi kwazo zonke izingxenye ezicutshunguliwe zamaqembu acutshungulwa ngokusebenzisa i-200mg/kg ye-diludine ayibonakali lapho i-1.79g yokudla okuphakelayo yengezwa ngokwesilinganiso; kodwa-ke, umehluko uba sobala kancane kancane kanye ne-diludine ekhulayo, futhi umehluko wesilinganiso sezinto ezibonakalayo neqanda phakathi kwezingxenye ezicutshungulwayo uyabonakala (p<0.05), futhi umphumela ulungile uma i-150mg / kg ye-diludine futhi i-1.25: i-1 encishiswa ku-10.36% (p<0.01) uma iqhathaniswa neqembu lereferensi. Kubonwa ngesilinganiso seqanda eliphukile lazo zonke izingxenye ezicutshungulwayo, izinga leqanda eliphukile (p<0.05) lingancishiswa lapho i-diludine yengezwe ekudleni kwansuku zonke; futhi iphesenti lamaqanda angajwayelekile liyancipha (p<0.05) kanye nokwanda kwe-diludine.

 

2.2 Umthelela wekhwalithi yamaqanda

Kubonwa kuThebula lesi-3, inkomba yokuma kweqanda kanye namandla adonsela phansi athize awathinteki (p>0.05) lapho i-diludine yengezwa ekudleni kwansuku zonke, futhi isisindo segobolondo sikhuphuka ngokukhula kwe-diludine yengezwe ekudleni kwansuku zonke, lapho izisindo zamagobolondo zikhuphuka ngo-10.58% kanye no-10.85% (p<0.0) amaqembu uma kuqhathaniswa ne-5 <0.0 ngokulandelanayo no-5 <0.0 ngokulandelana. I-200mg/kg ye-diludine iyengezwa; ukushuba kwegobolondo leqanda kuyanda ngokukhula kwe-diludine ekudleni kwansuku zonke, lapho ubukhulu begobolondo leqanda bukhuphuka ngo-13.89% (p<0.05) lapho i-100mg/kg ye-diludine yengezwa uma iqhathaniswa namaqembu ereferensi, futhi ubukhulu bamagobolondo amaqanda banda ngo-19.44% (p<0.0.0.70% 1% 1) kanye no-2% (p<0.01 0.70% 1% 1) futhi 200mg/kg kwengezwa. Iyunithi ye-Haugh (p<0.01) iba ngcono ngokusobala uma i-diludine yengezwa, okubonisa ukuthi i-diludine inomphumela wokugqugquzela ukuhlanganiswa kwe-albumen ewugqinsi yomhlophe kweqanda. I-diludine inomsebenzi wokuthuthukisa inkomba ye-yolk, kodwa umehluko awubonakali ngokusobala (p<0.05). Okuqukethwe kwe-cholesterol yesikhupha seqanda kuwo wonke amaqembu kunomehluko futhi kungancishiswa ngokusobala (p<0.05) ngemva kokwengeza u-150 no-200mg/kg we-diludine. Isisindo esihlobene sesikhupha seqanda sihlukile kwesinye ngenxa yamanani ahlukene e-diludine engeziwe, lapho izisindo ezihambisanayo zesikhupha seqanda zithuthukiswa zibe ngu-18.01% kanye no-14.92% (p<0.05) lapho u-150mg/kg no-200mg/kg uma kuqhathaniswa neqembu lereferensi; ngakho-ke, i-diludine efanelekile inomphumela wokukhuthaza ukuhlanganiswa kwe-yolk yeqanda.

 

Ithebula 3 Imiphumela ye-diludine kwikhwalithi yamaqanda

Inani le-diludine elizongezwa (mg/kg)
Ikhwalithi yeqanda	0	100	150	200
Inkomba yokuma kweqanda (%)
Amandla adonsela phansi aqondene neqanda (g/cm3)
Isisindo esihlobene segobolondo leqanda (%)
Ubukhulu begobolondo leqanda (mm)
Iyunithi ye-Haugh (U)
Inkomba yesikhupha seqanda (%)
I-Cholesterol yeqanda leqanda (%)
Isisindo esihlobene sesikhupha seqanda (%)

 

2.3 Imiphumela kumaphesenti amafutha esiswini kanye nokuqukethwe kwamafutha esibindi ezinkukhu ezizalelayo

Bheka Umfanekiso 1 kanye noMfanekiso 2 ngemiphumela ye-diludine kumaphesenti wamafutha asesiswini kanye nokuqukethwe kwamafutha esibindi ezinkukhu ezizalelayo.

 

 

 

Umfanekiso 1 Umthelela we-diludine kumaphesenti wamafutha e-bodominal (PAF) ezinkukhu ezizalelayo

 

	Iphesenti lamafutha e-bodominal
	Inani le-diludine elizongezwa

 

 

Umfanekiso 2 Umphumela we-diludine kumafutha esibindi (LF) ezinkukhu ezizalelayo

	Okuqukethwe kwamafutha esibindi
	Inani le-diludine elizongezwa

Kubonwa kuMdwebo 1, amaphesenti amafutha e-bodominal eqembu lokuhlola ancishiswa ngo-8.3% kanye no-12.11% (p<0.05) ngokulandelanayo lapho i-100 kanye ne-150mg/kg ye-diludine uma iqhathaniswa neqembu lereferensi, futhi iphesenti lamafutha e-bodominal lincishiswa ku-33.49% (p<0.01mg) lapho i-diludine ye-0.01 yengezwe ngu-0.01mg. Kubonwa kuMfanekiso 2, amafutha esibindi (ome ngokuphelele) acutshungulwe yi-100, 150, 200mg/kg ye-diludine ngokulandelana ancishiselwa ku-15.00% (p<0.05), 15.62% (p<0.05) kanye no-27.7% (p) <0 ngokulandelana; ngakho-ke, i-diludine inomphumela wokunciphisa iphesenti lamafutha e-bodominal kanye nokuqukethwe kwamafutha esibindi kokuqukethwe kokubeka ngokusobala, lapho umphumela ulungile lapho i-200mg / kg ye-diludine yengezwa.

2.4 Umphumela ku-serum biochemical index

Kubonwa kuThebula lesi-4, umehluko phakathi kwezingxenye ezicutshungulwe phakathi neSigaba I (30d) sokuhlolwa kwe-SOD awubonakali, futhi izinkomba ze-serum biochemical yawo wonke amaqembu lapho i-diludine yengezwe kuSigaba II (80d) sokuhlolwa ziphakeme kuneqembu lereferensi (p<0.05). I-uric acid (p<0.05) ku-serum ingancishiswa lapho kufakwa u-150mg/kg kanye no-200mg/kg we-diludine; kuyilapho umphumela (p<0.05) utholakala lapho i-100mg/kg ye-diludine yengezwa esigabeni I. I-diludine inganciphisa i-triglyceride ku-serum, lapho umphumela ulungile (p<0.01) eqenjini lapho i-150mg/kg ye-diludine yengezwe kuSigaba I, futhi ilungile eqenjini le-20kg/i-Phasedine uma i-20kg yengezwe ku-20kg ye-diludine. I-cholesterol ephelele ku-serum iyancipha ngokukhula kwe-diludine engezwe ekudleni kwansuku zonke, ikakhulukazi okuqukethwe kwe-cholesterol isiyonke ku-serum yehliswa ibe ngu-36.36% (p<0.01) no-40.74% (p<0.01) ngokulandelanayo lapho i-150mg/kg kanye ne-200mg/kg ye-diludine yengezwe esigabeni se-2% futhi yehliswa ku-0% yereferensi. (p<0.01), 37.40% (p<0.01) kanye no-46.66% (p<0.01) ngokulandelanayo lapho u-100mg/kg, 150mg/kg kanye no-200mg/kg we-diludine engezwa eSigabeni II uma kuqhathaniswa neqembu lereferensi. Ngaphezu kwalokho, i-ALP iyanda ngokukhula kwe-diludine engezwe ekudleni kwansuku zonke, kuyilapho amanani e-ALP eqenjini okufakwa kulo u-150mg/kg kanye no-200mg/kg we-diludine aphakeme kuneqembu lereferensi (p<0.05) ngokusobala.

Ithebula 4 Imiphumela ye-diludine kumapharamitha we-serum

Inani le-diludine elizongezwa (mg/kg) Esigabeni I (30d) sokuhlolwa
Into	0	100	150	200
I-Superoxide dismutase (mg/mL)
I-Uric acid
I-Triglyceride (mmol/L)
I-Lipase (U/L)
I-Cholesterol (mg/dL)
I-Glutamic-pyruvic transaminase (U/L)
I-Glutamic-oxalacetic transaminase (U/L)
I-alkaline phosphatase (mmol/L)
I-calcium ion (mmol/L)
I-Inorganic phosphorus (mg/dL)

 

Inani le-diludine elizongezwa (mg/kg) Esigabeni II (80d) sokuhlolwa
Into	0	100	150	200
I-Superoxide dismutase (mg/mL)
I-Uric acid
I-Triglyceride (mmol/L)
I-Lipase (U/L)
I-Cholesterol (mg/dL)
I-Glutamic-pyruvic transaminase (U/L)
I-Glutamic-oxalacetic transaminase (U/L)
I-alkaline phosphatase (mmol/L)
I-calcium ion (mmol/L)
I-Inorganic phosphorus (mg/dL)

 

3 Ukuhlaziya nengxoxo

3.1 I-diludine ekuhlolweni ithuthukise izinga lokuzala, isisindo seqanda, iyunithi ye-Haugh kanye nesisindo esilinganiselwe sesikhupha seqanda, okubonise ukuthi i-diludine ibe nemiphumela yokukhuthaza ukufana kweprotheni kanye nokwenza ngcono inani lokuhlanganiswa kwe-albumen ewugqinsi yeqanda elimhlophe kanye nephrotheni yesikhupha seqanda. Ngaphezu kwalokho, okuqukethwe kwe-uric acid ku-serum kwancishiswa ngokusobala; futhi ngokuvamile kwavunywa ukuthi ukuncishiswa kokuqukethwe kwe-nitrogen engeyona amaprotheni ku-serum kwakusho ukuthi isivinini se-catabolism samaprotheni sincishisiwe, futhi isikhathi sokugcinwa kwe-nitrogen sahlehliswa. Lo mphumela unikeze isisekelo sokwandisa ukugcinwa kwamaprotheni, ukukhuthaza ukubeka amaqanda kanye nokwenza ngcono isisindo seqanda lezinkukhu ezizalelayo. Umphumela wokuhlolwa uveze ukuthi umphumela wokubeka ulungile lapho kwengezwa i-150mg/kg ye-diludine, eyayihambisana nomphumela.^[6,7]ye-Bao Erqing ne-Qin Shangzhi futhi itholwe ngokungeza i-diludine ngesikhathi sekwephuzile sokubekela izinkukhu. Umphumela wehliswa lapho inani le-diludine lidlula i-150mg/kg, okungenzeka kungenxa yokuthi ukuguqulwa kwamaprotheni.^[8]kwathinteka ngenxa yomthamo oweqile kanye nomthwalo owedlulele we-metabolism yesitho ku-diludine.

3.2 Ukugxila kwe-Ca²⁺ku-serum yeqanda lokubekela yancipha, i-P ku-serum yancipha ekuqaleni futhi umsebenzi we-ALP wanda ngokusobala lapho kukhona i-diludine, okubonisa ukuthi i-diludine ithinte i-metabolism ye-Ca no-P ngokusobala. U-Yue Wenbin ubike ukuthi i-diludine ingakhuthaza ukumuncwa^[9] izakhi zamaminerali i-Fe ne-Zn; I-ALP yayikhona ikakhulukazi ezicutshini, njengesibindi, ithambo, umgudu wamathumbu, izinso, njll.; I-ALP ku-serum yayisuka esibindini nasethanjeni ikakhulukazi; I-ALP esethanjeni yayikhona e-osteoblast ikakhulukazi futhi yayikwazi ukuhlanganisa i-phosphate ion ne-Ca2 evela ku-serum ngemva kokuguqulwa ngokukhuthaza ukubola kwe-phosphate nokwandisa ukugcwala kwe-ion ye-phosphate, futhi yafakwa ethanjeni ngendlela ye-hydroxyapatite, njll. izinkomba. Ngaphezu kwalokho, izinga leqanda eliphukile kanye nephesenti leqanda elingavamile lancishiswa ngokusobala ngokusebenza kokubeka, okubuye kwachaza leli phuzu.

3.3 Ukufakwa kwamafutha esiswini kanye nokuqukethwe kwamafutha esibindi ezinkukhu ezizalelayo kwancishiswa ngokusobala ngokufaka i-diludine ekudleni, okubonisa ukuthi i-diludine ibe nomthelela wokuvimbela ukuhlanganiswa kwamafutha emzimbeni. Ngaphezu kwalokho, i-diludine ingathuthukisa umsebenzi we-lipase ku-serum ekuqaleni kwesigaba; umsebenzi we-lipase wanda ngokusobala eqenjini lapho i-100mg / kg ye-diludine yanezelwa, futhi okuqukethwe kwe-triglyceride ne-cholesterol ku-serum kwancishiswa (p<0.01), okubonisa ukuthi i-diludine ingakhuthaza ukubola kwe-triglyceride futhi ivimbele ukuhlanganiswa kwe-cholesterol. Ukufakwa kwamafutha kungavinjelwa ngenxa yokuthi i-enzyme ye-lipid metabolism esibindini^[10,11], kanye nokuncipha kwe-cholesterol esitokisini seqanda nakho kwachaza leli phuzu [13]. U-Chen Jufang ubike ukuthi i-diludine inganqanda ukwakheka kwamafutha esilwaneni futhi ithuthukise amaphesenti enyama ezondile yama-broilers nengulube, futhi ibe nomthelela wokwelapha isibindi esinamafutha. Umphumela wokuhlolwa wawucacisa lo mshini wokusebenza, futhi imiphumela yokuhlakaza kanye nokubhekwa kwezinkukhu zokuhlola iphinde yafakazela ukuthi i-diludine inganciphisa izinga lokuvela kwesibindi esinamafutha ezinkukhu ezibekela amaqanda ngokusobala.

3.4 I-GPT ne-GOT yizinkomba ezimbili ezibalulekile ezibonisa ukusebenza kwesibindi nenhliziyo, futhi isibindi nenhliziyo kungase konakaliswe uma imisebenzi yako iphezulu kakhulu. Imisebenzi ye-GPT kanye ne-GOT ku-serum ayizange ishintshwe ngokusobala lapho i-diludine yengezwa ekuhlolweni, okubonisa ukuthi isibindi nenhliziyo akukonakali; ngokuqhubekayo, umphumela wokulinganisa we-SOD ubonise ukuthi umsebenzi we-SOD ku-serum ungathuthukiswa ngokusobala lapho i-diludine isetshenziswa isikhathi esithile. I-SOD ibhekisela ku-scavenger omkhulu we-superoxide free radical emzimbeni; kubaluleke kakhulu ukugcina ubuqotho be-membrane ye-biological, ukuthuthukisa amandla okuzivikela omzimba kanye nokugcina impilo yesilwane lapho okuqukethwe kwe-SOD emzimbeni kwanda. I-Quh Hai, njll. ibike ukuthi i-diludine ingathuthukisa umsebenzi we-6-glucose phosphate dehydrogenase kulwelwesi lwebhayoloji futhi iqinise izicubu [2] zeseli yebhayoloji. U-Sniedze uveze ukuthi i-diludine inqande umsebenzi [4] we-NADPH cytochrome C reductase ngokusobala ngemva kokutadisha ubudlelwano phakathi kwe-diludine kanye ne-enzyme efanelekile ku-NADPH ethize yokudluliswa kwama-electron ku-microsome yesibindi segundane. Ama-odydents aphinde aveza ukuthi i-diludine yayihlobene [4] nesistimu ye-oxidase eyinhlanganisela kanye ne-enzyme ye-microsomal ehlobene ne-NADPH; kanye nendlela yesenzo se-diludine ngemva kokungena esilwaneni ukudlala indima yokumelana ne-oxidation nokuvikela ulwelwesi lwebhayoloji [8] ngokunqamula umsebenzi we-electron yokudlulisa i-enzyme ye-NADPH ye-microsome futhi ivimbele inqubo ye-peroxidation ye-lipid compound. Umphumela wokuhlola wafakazela ukuthi umsebenzi wokuvikela we-diludine kuya kulwelwesi lwebhayoloji usuka ezinguqukweni zomsebenzi we-SOD uye ezinguqukweni zemisebenzi ye-GPT ne-GOT futhi wafakazela imiphumela yocwaningo ye-Sniedze ne-Odydents.

 

Ireferensi

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2 Qu Hai, Lv Ye, Wang Baosheng, Umphumela we-diludine wengezwe ekudleni kwansuku zonke esilinganisweni sokukhulelwa kanye nekhwalithi yesidoda senyama kanogwaja.J. Chinese Journal of Rabbit Farming1994(6): 6-7

3 Chen Jufang, Yin Yuejin, Liu Wanhan, njll. Ukuhlolwa kokusetshenziswa okunwetshiwe kwe-diludine njengesengezo sokuphakelayoOkuphakelayo Ucwaningo1993 (3): 2-4

4 U-Zheng Xiaozhong, u-Li Kelu, u-Yue Wenbin, njll. Ingxoxo yomphumela wesicelo kanye nendlela yokusebenza ye-diludine njengomgqugquzeli wokukhulisa izinkukhu.Okuphakelayo Ucwaningo1995 (7): 12-13

5 Chen Jufang, Yin Yuejin, Liu Wanhan, njll. Ukuhlolwa kokusetshenziswa okunwetshiwe kwe-diludine njengesengezo sokuphakelayoOkuphakelayo Ucwaningo1993 (3): 2-5

6 I-Bao Erqing, i-Gao Baohua, Ukuhlolwa kwe-diludine yokondla uhlobo lwedada lasePekingOkuphakelayo Ucwaningo1992 (7): 7-8

Isivivinyo se-7 Qin Shangzhi sokuthuthukisa umkhiqizo wezinkukhu zohlobo lwenyama ngasekupheleni kwesikhathi sokubekela ngokusebenzisa i-diludineI-Guangxi Journal of Animal Husbandry & Veterinary Medicine1993.9(2): 26-27

8 Dibner J Jl Lvey FJ ​​Amaprotheni esibindi kanye ne-amino acid metabolian ezinkukhu Izinkukhu Sci1990.69(7): 1188- 1194

9 U-Yue Wenbin, u-Zhang Jianhong, u-Zhao Peie, njll. Ucwaningo lokwengezwa kwe-diludine kanye nokulungiswa kwe-Fe-Zn ekudleni kwansuku zonke kwezinkukhu ezizalelayoOkuphakelayo Nemfuyo1997, 18(7): 29-30

10 Mildner A na M, Steven D Clarke Porcine fatty acid synthase cloning ye-DNA ehambisanayo, ukusatshalaliswa kwezicubu ze-itsmRNA kanye nokucindezelwa kwenkulumo nge-somatotropin kanye nephrotheni yokudla u-J Nutri 1991, 121 900

11 W alzon RL Smon C, M orishita T, et a I Fatty liver hemorrhagic syndrome ezikhukhukazini ezidliwe ukudla okuhlanzekile Imisebenzi ekhethiwe ye-enzyme kanye ne-histology yesibindi maqondana nokuhlonishwa kwesibindi nokusebenza kokuzalaIzinkukhu Sci,1993 72(8): 1479- 1491

12 UDonaldson WE I-lipid metabolism esibindini samatshwele asabela ekunceleniIzinkukhu Sci. 1990, 69(7) : 1183- 1187

13 Ksiazk ieu icz J. K ontecka H, ​​H ogcw sk i L Inothi le-cholesterol yegazi njengenkomba yokukhuluphala komzimba kumadadaIjenali ye-Aninal and Feed Science,1992, 1(3/4): 289- 294