IsifinyezoUkuhlolwa kwenziwe ukuze kufundwe imiphumela ye-diludine ekusebenzeni kahle kokubeka amaqanda kanye nekhwalithi yamaqanda ezinkukhu kanye nendlela yokusebenza kwemiphumela ngokunquma izinkomba zamapharamitha amaqanda kanye ne-serum. Izinkukhu ze-ROM ezingu-1024 zahlukaniswa ngamaqembu amane ngalinye elalihlanganisa amakhophi amane ezinkukhu ezingu-64 ngasinye, Amaqembu okwelapha athola ukudla okufanayo okuyisisekelo okunezelwe nge-0, 100, 150, 200 mg/kg diludine ngokulandelana izinsuku ezingu-80. Imiphumela yayimi kanje. Ukwengezwa kwe-diludine ekudleni kuthuthukise ukusebenza kokubeka amaqanda ezinkukhu, lapho ukwelashwa okungu-150 mg/kg kwakungcono kakhulu; izinga layo lokubeka amaqanda lenyuswe ngo-11.8% (p< 0.01), ukuguqulwa kwesisindo samaqanda kwehle ngo-10.36% (p< 0 01). Isisindo samaqanda sanda ngokwanda kwe-diludine. I-Diludine yehlise kakhulu ukuhlushwa kwe-uric acid egazini (p< 0.01); ukwengeza i-diludine kwehlise kakhulu i-serum Ca2.2+kanye nokuqukethwe kwe-phosphate okungaphili, kanye nomsebenzi owandisiwe we-alkine phosphatase (ALP) ye-serum (p< 0.05), ngakho-ke kwaba nemiphumela ebalulekile ekunciphiseni ukuphuka kweqanda (p<0.05) kanye nokungajwayelekile (p< 0.05); i-diludine yandise kakhulu ukuphakama kwe-albhamuen. Inani le-Haugh (p<0.01), ukujiya kwegobolondo kanye nesisindo segobolondo (p< 0.05), i-150 kanye ne-200mg/kg i-diludine nayo yehlise i-choleasterol iyonke ku-egg yolk (p< 0 05), kodwa yandise isisindo se-egg yolk (p< 0.05). Ngaphezu kwalokho, i-diludine ingathuthukisa umsebenzi we-lipase (p< 0.01), futhi yehlise okuqukethwe kwe-triglyceride (TG3) (p<0.01) kanye ne-cholesterol (CHL) (p< 0 01) ku-serum, yehlise iphesenti lamafutha esiswini (p< 0.01) kanye nokuqukethwe kwamafutha esibindi (p< 0.01), yayinamandla okuvimbela izinkukhu esibindini esinamafutha. I-Diludine yandise kakhulu umsebenzi we-SOD ku-serum (p<0 01) lapho ingezwa ekudleni isikhathi esingaphezu kwezinsuku ezingama-30. Nokho, akukho mehluko obalulekile otholakale emisebenzini ye-GPT kanye ne-GOT ye-serum phakathi kweqembu lokulawula nelashwayo. Kwaphethwa ngokuthi i-diludine ingavimbela ulwelwesi lwamaseli ekungcolisweni.
Amagama ayisihluthuleloi-Diludine; inkukhu; i-SOD; i-cholesterol; i-triglyceride, i-lipase
I-diludine iyisengezo esisha samavithamini esingezona ezondlo esilwa nokubola futhi inemiphumela[1-3]yokuvimbela ukungcoliswa kwe-membrane yezinto eziphilayo kanye nokuqinisa izicubu zamaseli ezinto eziphilayo, njll. Ngawo-1970, uchwepheshe wezolimo waseLatvia endaweni eyayiyiSoviet Union wathola ukuthi i-diludine yayinemiphumela[4]yokukhuthaza ukukhula kwezinkukhu nokumelana nokubanda nokuguga kwezinye izitshalo. Kwabikwa ukuthi i-diludine ayigcini nje ngokukhuthaza ukukhula kwesilwane, kodwa futhi ithuthukisa ukusebenza kokuzala kwesilwane ngokusobala futhi ithuthukise izinga lokukhulelwa, umkhiqizo wobisi, umkhiqizo wamaqanda kanye nezinga lokuchamisela kwesilwane sesifazane.[1, 2, 5-7]Ucwaningo lwe-diludine eShayina lwaqalwa kusukela ngawo-1980, futhi iningi lezifundo mayelana ne-diludine eShayina zikhawulelwe ekusebenziseni kuze kube manje, futhi kubikwa izivivinyo ezimbalwa zokubekela izinyoni. UChen Jufang (1993) wabika ukuthi i-diludine ingathuthukisa umkhiqizo weqanda kanye nesisindo seqanda lenkukhu, kodwa ayizange ijule.[5]isifundo sendlela yokusebenza kwayo. Ngakho-ke, siqalise isifundo esihlelekile somphumela kanye nendlela esebenza ngayo ngokupha izinkukhu ezizalelayo ukudla okufakwe i-diludine, futhi ingxenye eyodwa yomphumela manje ibikwa kanje:
Ithebula 1 Ukwakheka kanye nezingxenye zezakhamzimba zokudla kokuhlola
%
-- ...
Ukwakheka kokudla Izakhi ze-Nutrient
-- ...
Ummbila 62 ME③ 11.97
Inhlama yobhontshisi 20 CP 17.8
Ukudla kwenhlanzi 3 Ca 3.42
Ukudla kwe-rapeseed 5 P 0.75
Ukudla kwamathambo 2 M et 0.43
Isidlo samatshe 7.5 M kanye ne-Cys 0.75
I-Methionine 0.1
Usawoti 0.3
Amavithamini amaningi① 10
Izinto zokulandelela② 0.1
-- ...
① Amavithamini amaningi: 11mg we-riboflavin, 26mg we-folic acid, 44mg we-oryzanin, 66mg we-niacin, 0.22mg we-biotin, 66mg we-B6, 17.6ug we-B12, 880mg we-choline, 30mg we-VK, 66IU we-VE, 6600ICU ye-VDkanye ne-20000ICU ye-VA, zengezwa kukhilogremu ngayinye yokudla; kanti ama-multivitamin angu-10g afakwa ekudleni ngakunye okungu-50kg.
② Izinto ezilandelanayo (mg/kg): 60 mg we-Mn, 60 mg we-Zn, 80 mg we-Fe, 10 mg we-Cu, 0.35 mg we-I kanye no-0.3 mg we-Se zengezwa kukhilogremu ngayinye yokudla.
③ Iyunithi yamandla aguqulwayo ibhekisela ku-MJ/kg.
1. Izinto zokwakha kanye nendlela
1.1 Izinto zokuhlola
I-Beijing Sunpu Biochem. & Tech. Co., Ltd. kufanele inikeze i-diludine; futhi isilwane sokuhlola kufanele sibhekisele ezinkukhu zaseRoma ezizalela amaqanda ezithengiswayo ezinezinsuku ezingu-300 ubudala.
Ukudla kokuhlola: ukudla kokuhlola kufanele kulungiselelwe ngokwesimo sangempela ngesikhathi sokukhiqiza ngokusekelwe ku-NRC standard, njengoba kuboniswe kuThebula 1.
1.2 Indlela yokuhlola
1.2.1 Ukuhlolwa kokondla: ukuhlolwa kokondla kufanele kuqaliswe epulazini leNkampani yaseHongji eJiande City; Izinkukhu zaseRoma eziyi-1024 ezizalela amaqanda kufanele zikhethwe futhi zihlukaniswe ngamaqembu amane ngokungahleliwe futhi ngayinye ibe yizicucu ezingama-256 (iqembu ngalinye kufanele liphindwe izikhathi ezine, kanti inkukhu ngayinye kufanele iphindwe izikhathi ezingama-64); izinkukhu kufanele zondliwe ngokudla okune okunokuqukethwe okuhlukile kwe-diludine, kanye nokudla okungu-0, 100, 150, 200mg/kg kufanele kwengezwe eqenjini ngalinye. Ukuhlolwa kwaqalwa ngo-Ephreli 10, 1997; futhi izinkukhu zazingathola ukudla futhi ziphuze amanzi ngokukhululeka. Ukudla okuthathwe yiqembu ngalinye, izinga lokubeka amaqanda, umkhiqizo weqanda, iqanda eliphukile kanye nenani leqanda elingavamile kufanele kubhalwe phansi. Ngaphezu kwalokho, ukuhlolwa kwaphela ngoJuni 30, 1997.
1.2.2 Ukulinganiswa kwekhwalithi yeqanda: Amaqanda angu-20 kufanele athathwe ngokungahleliwe lapho kuhlolwa kwenziwa izinsuku ezine ezingama-40 ukuze kulinganiswe izinkomba ezihlobene nekhwalithi yeqanda, njengenkomba yokuma kweqanda, iyunithi ye-haugh, isisindo esilinganiselwe segobolondo, ubukhulu begobolondo, inkomba ye-yolk, isisindo esilinganiselwe se-yolk, njll. Ngaphezu kwalokho, okuqukethwe yi-cholesterol ku-yolk kufanele kulinganiswe ngokusebenzisa indlela ye-COD-PAP phambi kwe-reagent ye-Cicheng ekhiqizwe yi-Ningbo Cixi Biochemical Test Plant.
1.2.3 Ukulinganiswa kwe-serum biochemical index: Izinkukhu zokuhlola eziyi-16 kufanele zithathwe eqenjini ngalinye isikhathi ngasinye lapho ukuhlolwa kwenziwa izinsuku ezingama-30 futhi lapho ukuhlolwa sekuqediwe ukuze kulungiswe i-serum ngemva kokusampula igazi emthanjeni osephikweni. I-serum kufanele igcinwe ezingeni lokushisa eliphansi (-20℃) ukuze kulinganiswe izinkomba ze-biochemical ezifanele. Iphesenti lamafutha esiswini kanye nokuqukethwe kwamafutha esibindi kufanele kulinganiswe ngemva kokuhlatshwa nokukhishwa kwamafutha esiswini kanye nesibindi lapho kuqediwe ukusampula igazi.
I-superoxide dismutase (i-SOD) kufanele ikalwe ngokusebenzisa indlela yokugcwala phambi kwekhithi ye-reagent ekhiqizwe yi-Beijing Huaqing Biochem. & Tech. Research Institute. I-uric acid (UN) esegazini kufanele ikalwe ngokusebenzisa indlela ye-U ricase-PAP phambi kwekhithi ye-Cicheng reagent; i-triglyceride (TG3) kufanele ikalwe ngokusebenzisa indlela ye-GPO-PAP enesinyathelo esisodwa phambi kwekhithi ye-Cicheng reagent; i-lipase kufanele ikalwe ngokusebenzisa i-nephelometry phambi kwekhithi ye-Cicheng reagent; i-cholesterol iyonke esegazini (i-CHL) kufanele ikalwe ngokusebenzisa indlela ye-COD-PAP phambi kwekhithi ye-Cicheng reagent; i-glutamic-pyruvic transaminase (GPT) kufanele ikalwe ngokusebenzisa i-colorimetry phambi kwekhithi ye-Cicheng reagent; i-glutamic-oxalacetic transaminase (GOT) kufanele ikalwe ngokusebenzisa i-colorimetry phambi kwekhithi ye-Cicheng reagent; i-alkaline phosphatase (ALP) kufanele ikalwe ngokusebenzisa indlela yesilinganiso phambi kwekhithi ye-Cicheng reagent; i-calcium ion (Ca2+) ku-serum kufanele kulinganiswe ngokusebenzisa i-methylthymol blue complex. Indlela eyodwa phambi kwe-Cicheng reagent kit; i-phosphorus engaphili (P) kufanele ilinganiswe ngokusebenzisa indlela ye-molybdate blue phambi kwe-Cicheng reagent kit.
2 Umphumela wokuhlolwa
2.1 Umphumela ekusebenzeni kahle kokubeka
Ukusebenza kokubeka kwamaqembu ahlukene acutshungulwa ngokusebenzisa i-diludine kuboniswe kuThebula 2.
Ithebula 2 Ukusebenza kwezinkukhu eziphakelwa ukudla okuyisisekelo okunezinga ezine ze-diludine
| Inani le-diludine okufanele lifakwe (mg/kg) | ||||
| 0 | 100 | 150 | 200 | |
| Ukudla okudliwayo (g) |  | |||
| Izinga lokubeka (%) | ||||
| Isisindo esimaphakathi seqanda (g) | ||||
| Isilinganiso sezinto ezibonakalayo neqanda | ||||
| Izinga lamaqanda aphukile (%) | ||||
| Izinga leqanda elingavamile (%) | ||||
Kusukela kuThebula 2, amazinga okubekwa kwawo wonke amaqembu acutshungulwa ngokusebenzisa i-diludine athuthukisiwe ngokusobala, lapho umphumela lapho ucutshungulwa ngokusebenzisa i-150mg/kg ungcono kakhulu (kufika ku-83.36%), kanti u-11.03% (p<0.01) uthuthukisiwe uma kuqhathaniswa neqembu elibhekiselwe kulo; ngakho-ke i-diludine inomphumela wokuthuthukisa izinga lokubekwa. Uma kubhekwa isisindo esimaphakathi seqanda, isisindo seqanda siyanda (p>0.05) ngokunyuka kwe-diludine ekudleni kwansuku zonke. Uma kuqhathaniswa neqembu elibhekiselwe kulo, umehluko phakathi kwazo zonke izingxenye ezicutshungulwa zamaqembu acutshungulwa ngokusebenzisa i-200mg/kg ye-diludine awubonakali lapho u-1.79g wokudla kokudla ungezwa ngokwesilinganiso; Nokho, umehluko uba sobala kancane kancane kanye nokwanda kwe-diludine, futhi umehluko wesilinganiso sezinto ezisetshenziswayo neqanda phakathi kwezingxenye ezicutshungulwayo usobala (p<0.05), futhi umphumela uba ngcono kakhulu uma i-diludine ingu-150mg/kg futhi ingu-1.25:1 encishisiwe ngo-10.36% (p<0.01) uma kuqhathaniswa neqembu elibhekiselwe kulo. Uma kubhekwa izinga lamaqanda aphukile lazo zonke izingxenye ezicutshungulwayo, izinga lamaqanda aphukile (p<0.05) lingancishiswa uma i-diludine ingezwa ekudleni kwansuku zonke; futhi iphesenti lamaqanda angavamile lincishisiwe (p<0.05) ngokukhula kwe-diludine.
2.2 Umphumela kwikhwalithi yeqanda
Njengoba kuboniswe kuThebula 3, inkomba yokuma kweqanda kanye nobukhulu beqanda elithile akuthinteki (p>0.05) lapho i-diludine ingezwa ekudleni kwansuku zonke, futhi isisindo segobolondo siyanda ngokunyuka kwe-diludine engezwa ekudleni kwansuku zonke, lapho isisindo samagobolondo sikhuphuka ngo-10.58% kanye no-10.85% (p<0.05) ngokulandelana uma kuqhathaniswa namaqembu okubhekisela lapho kufakwa u-150 no-200mg/kg we-diludine; ukujiya kwegobolondo leqanda kuyanda ngokunyuka kwe-diludine ekudleni kwansuku zonke, lapho ukujiya kwegobolondo leqanda kuyanda ngo-13.89% (p<0.05) lapho kufakwa u-100mg/kg we-diludine uma kuqhathaniswa namaqembu okubhekisela, futhi ukujiya kwamagobolondo amaqanda kuyanda ngo-19.44% (p<0.01) kanye no-27.7% (p<0.01) ngokulandelana lapho kufakwa u-150 no-200mg/kg. Iyunithi ye-Haugh (p<0.01) ithuthukiswa ngokusobala uma i-diludine ingezwa, okubonisa ukuthi i-diludine inomphumela wokukhuthaza ukwenziwa kwe-albumen ejiyile yeqanda elimhlophe. I-diludine inomsebenzi wokuthuthukisa inkomba ye-yolk, kodwa umehluko awusobala (p<0.05). Okuqukethwe yi-cholesterol yeqanda elimhlophe lamaqembu wonke kuyahluka futhi kungancishiswa ngokusobala (p<0.05) ngemuva kokufaka u-150 no-200mg/kg we-diludine. Isisindo esilinganiselwe seqanda elimhlophe sihlukile komunye nomunye ngenxa yenani elihlukile le-diludine elingeziwe, lapho isisindo esilinganiselwe seqanda elimhlophe sithuthukiswa ngo-18.01% no-14.92% (p<0.05) lapho u-150mg/kg no-200mg/kg uma kuqhathaniswa neqembu elibhekiselwe kulo; ngakho-ke, i-diludine efanele inomphumela wokukhuthaza ukwenziwa kweqanda elimhlophe.
Ithebula 3 Imiphumela ye-diludine kwikhwalithi yamaqanda
| Inani le-diludine okufanele lifakwe (mg/kg) | ||||
| Ikhwalithi yeqanda | 0 | 100 | 150 | 200 |
| Inkomba yokuma kweqanda (%) |  | |||
| Ubunzima obuthile beqanda (g/cm3) | ||||
| Isisindo esilinganiselwe segobolondo leqanda (%) | ||||
| Ubukhulu begobolondo leqanda (mm) | ||||
| Iyunithi yokuhleka (U) | ||||
| Inkomba yeqanda (%) | ||||
| I-cholesterol yeqanda eliphuzi (%) | ||||
| Isisindo esilinganiselwe seqanda eliphuzi (%) | ||||
2.3 Imiphumela kumaphesenti amafutha esiswini kanye nokuqukethwe kwamafutha esibindi sezinkukhu ezizalelayo
Bheka uMfanekiso 1 kanye noMfanekiso 2 ukuthola imiphumela ye-diludine kumaphesenti amafutha esiswini kanye nokuqukethwe kwamafutha esibindi ezinkukhu ezizalelayo.
Isithombe 1 Umphumela we-diludine kumaphesenti amafutha esisu (i-PAF) ezinkukhu ezizalelayo
| Amaphesenti amafutha esiswini | |
| Inani le-diludine elizongezwa |
Umfanekiso 2 Umphumela we-diludine kokuqukethwe kwamafutha esibindi (LF) kwezinkukhu ezizalelayo
| Okuqukethwe kwamafutha esibindi | |
| Inani le-diludine elizongezwa |
Kubonwe kusukela kuMfanekiso 1, amaphesenti amafutha esiswini eqembu lokuhlola ancishisiwe ngo-8.3% kanye no-12.11% (p<0.05) ngokulandelana lapho u-100 no-150mg/kg we-diludine uma kuqhathaniswa neqembu elibhekiselwe kulo, kanti iphesenti lamafutha esiswini lincishisiwe ngo-33.49% (p<0.01) lapho kufakwa u-200mg/kg we-diludine. Kubonwe kusukela kuMfanekiso 2, okuqukethwe kwamafutha esibindi (okomile ngokuphelele) kucutshungulwe ngo-100, 150, 200mg/kg we-diludine ngokulandelana kuncishisiwe ngo-15.00% (p<0.05), 15.62% (p<0.05) kanye no-27.7% (p<0.01) ngokulandelana uma kuqhathaniswa neqembu elibhekiselwe kulo; Ngakho-ke, i-diludine inomphumela wokunciphisa iphesenti lamafutha esiswini kanye nokuqukethwe kwamafutha esibindi kokuqukethwe kokulala ngokusobala, lapho umphumela uba ngcono kakhulu uma kufakwa i-200mg/kg ye-diludine.
2.4 Umphumela ku-serum biochemical index
Njengoba kuboniswe kuThebula 4, umehluko phakathi kwezingxenye ezicutshungulwe ngesikhathi seSigaba I (30d) sokuhlolwa kwe-SOD awubonakali, futhi izinkomba ze-biochemical ze-serum zawo wonke amaqembu lapho i-diludine ingezwe khona eSigabeni II (80d) sokuhlolwa ziphakeme kuneqembu elibhekiselwe kulo (p<0.05). I-uric acid (p<0.05) esesilinganisweni ingancishiswa uma kufakwa u-150mg/kg kanye no-200mg/kg we-diludine; kuyilapho umphumela (p<0.05) utholakala uma kufakwa u-100mg/kg we-diludine eSigabeni I. I-diludine inganciphisa i-triglyceride esesilinganisweni, lapho umphumela uba ngcono kakhulu (p<0.01) eqenjini lapho kufakwa u-150mg/kg we-diludine eSigabeni I, futhi uba ngcono kakhulu eqenjini lapho kufakwa u-200mg/kg we-diludine eSigabeni II. I-cholesterol iyonke esegazini iyancipha ngokunyuka kwe-diludine efakwa ekudleni kwansuku zonke, ikakhulukazi okuqukethwe yi-cholesterol iyonke esegazini kwehla ngo-36.36% (p<0.01) kanye no-40.74% (p<0.01) ngokulandelana lapho kufakwa i-150mg/kg kanye ne-200mg/kg ye-diludine eSigabeni I uma kuqhathaniswa neqembu elibhekiselwe kulo, futhi kwehla ngo-26.60% (p<0.01), 37.40% (p<0.01) kanye no-46.66% (p<0.01) ngokulandelana lapho kufakwa i-100mg/kg, 150mg/kg kanye ne-200mg/kg ye-diludine eSigabeni II uma kuqhathaniswa neqembu elibhekiselwe kulo. Ngaphezu kwalokho, i-ALP iyanda ngokufaka i-diludine ekudleni kwansuku zonke, kuyilapho amanani e-ALP eqenjini lapho kufakwa khona i-150mg/kg kanye ne-200mg/kg ye-diludine aphezulu kuneqembu elibhekiselwe kulo (p<0.05) ngokusobala.
Ithebula 4 Imiphumela ye-diludine kumapharamitha e-serum
| Inani le-diludine elizongezwa (mg/kg) ku-Isigaba I (izinsuku ezingu-30) sokuhlolwa | ||||
| Into | 0 | 100 | 150 | 200 |
| I-Superoxide dismutase (mg/mL) |  | |||
| I-asidi ye-uric | ||||
| I-Triglyceride (mmol/L) | ||||
| I-Lipase (U/L) | ||||
| I-Cholesterol (mg/dL) | ||||
| I-transaminase ye-Glutamic-pyruvic (U/L) | ||||
| I-transaminase ye-Glutamic-oxalacetic (U/L) | ||||
| I-alkaline phosphatase (mmol/L) | ||||
| I-calcium ion (mmol/L) | ||||
| I-phosphorus engaphili (mg/dL) | ||||
| Inani le-diludine elizongezwa (mg/kg) ku-Phase II (80d) yokuhlolwa | ||||
| Into | 0 | 100 | 150 | 200 |
| I-Superoxide dismutase (mg/mL) |  | |||
| I-asidi ye-uric | ||||
| I-Triglyceride (mmol/L) | ||||
| I-Lipase (U/L) | ||||
| I-Cholesterol (mg/dL) | ||||
| I-transaminase ye-Glutamic-pyruvic (U/L) | ||||
| I-transaminase ye-Glutamic-oxalacetic (U/L) | ||||
| I-alkaline phosphatase (mmol/L) | ||||
| I-calcium ion (mmol/L) | ||||
| I-phosphorus engaphili (mg/dL) | ||||
3 Ukuhlaziywa kanye nengxoxo
3.1 I-diludine esivivinyweni ithuthukise izinga lokubekwa, isisindo seqanda, iyunithi ye-Haugh kanye nesisindo esilinganiselwe se-yolk yeqanda, okwabonisa ukuthi i-diludine yayinemiphumela yokukhuthaza ukumuncwa kweprotheni nokuthuthukisa inani lokwenziwa kwe-albumen ejiyile yeqanda elimhlophe kanye neprotheni ye-yolk yeqanda. Ngaphezu kwalokho, okuqukethwe kwe-uric acid ku-serum kwancishiswa ngokusobala; futhi ngokuvamile kwavunywa ukuthi ukwehla kokuqukethwe kwe-nitrogen engeyona iphrotheni ku-serum kusho ukuthi ijubane le-catabolism leprotheni lancishiswa, futhi isikhathi sokugcinwa kwe-nitrogen sahlehliswa. Lo mphumela wanikeza isisekelo sokwandisa ukugcinwa kwamaprotheni, ukukhuthaza ukubekwa kwamaqanda nokuthuthukisa isisindo seqanda lezinkukhu ezibeka amaqanda. Umphumela wokuhlolwa ukhombe ukuthi umphumela wokubekwa ungcono kakhulu lapho kungezwa i-150mg/kg ye-diludine, okwakuhambisana nomphumela.[6,7]kaBao Erqing noQin Shangzhi futhi yatholakala ngokungeza i-diludine ngesikhathi sokuphela kwezinkukhu ezizalelayo. Umphumela wehliswa lapho inani le-diludine lidlula u-150mg/kg, okungenzeka ukuthi kungenxa yokuguqulwa kwamaprotheni[8]yathinteka ngenxa yomthamo oweqile kanye nomthwalo oweqile wokuguqulwa kwesitho sibe yi-diludine.
3.2 Ukuhlushwa kwe-Ca2+ku-serum yeqanda elizalelayo kwehlisiwe, i-P ku-serum yehlisiwe ekuqaleni kanti umsebenzi we-ALP wanda ngokusobala lapho kukhona i-diludine, okubonisa ukuthi i-diludine ithinte ukusebenza kwe-Ca kanye ne-P ngokusobala. U-Yue Wenbin ubike ukuthi i-diludine ingakhuthaza ukumuncwa.[9] izakhi zamaminerali i-Fe ne-Zn; I-ALP yayikhona kakhulu ezicutshini, njengesibindi, ithambo, umgudu wamathumbu, izinso, njll.; I-ALP esesilinganisweni yayivela esibindini nasethanjeni ikakhulukazi; I-ALP esethanjeni yayikhona kakhulu e-osteoblast futhi yayingahlanganisa i-phosphate ion ne-Ca2 esesilinganisweni ngemva kokuguqulwa ngokukhuthaza ukubola kwe-phosphate nokwandisa ukuhlushwa kwe-phosphate ion, futhi yafakwa ethanjeni ngesimo se-hydroxyapatite, njll. ukuze kuholele ekunciphiseni kwe-Ca ne-P esesilinganisweni, okuhambisana nokwanda kobukhulu begobolondo leqanda kanye nesisindo esilinganiselwe segobolondo leqanda ezinkomba zekhwalithi yeqanda. Ngaphezu kwalokho, izinga leqanda eliphukile kanye nephesenti leqanda elingajwayelekile kwancishiswa ngokusobala ngokuya ngokusebenza kokubeka, okuchaze naleli phuzu.
3.3 Ukufakwa kwamafutha esiswini kanye nokuqukethwe kwamafutha esibindi ezinkukhu ezizalelayo kuncishisiwe ngokufaka i-diludine ekudleni, okubonisa ukuthi i-diludine yayinomphumela wokuvimbela ukwakheka kwamafutha emzimbeni. Ngaphezu kwalokho, i-diludine ingathuthukisa umsebenzi we-lipase ku-serum esigabeni sokuqala; umsebenzi we-lipase wanda ngokusobala eqenjini lapho kwanezelwa khona i-100mg/kg ye-diludine, futhi okuqukethwe kwe-triglyceride kanye ne-cholesterol ku-serum kuncishisiwe (p<0.01), okubonisa ukuthi i-diludine ingakhuthaza ukubola kwe-triglyceride futhi ivimbele ukwakheka kwe-cholesterol. Ukufakwa kwamafutha kungavinjelwa ngoba i-enzyme ye-lipid metabolism esibindini[10,11], kanye nokwehla kwe-cholesterol eqandeni leqanda nakho kuchaze leli phuzu [13]. UChen Jufang ubike ukuthi i-diludine ingavimbela ukwakheka kwamafutha esilwaneni futhi ithuthukise iphesenti lenyama engenamafutha ezinkukhu kanye nengulube, futhi yayinomphumela wokwelapha isibindi esinamafutha. Umphumela wokuhlolwa ucacise le ndlela yokusebenza, futhi imiphumela yokuhlukaniswa kanye nokubonwa kwezinkukhu zokuhlola nayo ifakazele ukuthi i-diludine inganciphisa izinga lokuvela kwesibindi esinamafutha ezinkukhu ezizalela ngokusobala.
3.4 I-GPT ne-GOT ziyizinkomba ezimbili ezibalulekile ezibonisa imisebenzi yesibindi nenhliziyo, kanti isibindi nenhliziyo kungase kulinyazwe uma imisebenzi yaso iphezulu kakhulu. Imisebenzi ye-GPT ne-GOT ku-serum ayizange ishintshwe ngokusobala lapho i-diludine ingezwa esivivinyweni, okwabonisa ukuthi isibindi nenhliziyo akulimalanga; ngaphezu kwalokho, umphumela wokulinganisa we-SOD ukhombisile ukuthi umsebenzi we-SOD ku-serum ungathuthukiswa ngokusobala lapho i-diludine isetshenziswa isikhathi esithile. I-SOD ibhekisela kumhluzi omkhulu we-superoxide free radical emzimbeni; ibaluleke kakhulu ekugcineni ubuqotho be-membrane yezinto eziphilayo, ukuthuthukisa ikhono lokuzivikela komzimba kanye nokugcina impilo yesilwane lapho okuqukethwe kwe-SOD emzimbeni kukhuphukile. U-Quh Hai, njll. ubike ukuthi i-diludine ingathuthukisa umsebenzi we-6-glucose phosphate dehydrogenase ku-membrane yezinto eziphilayo futhi izinzise izicubu [2] zeseli lezinto eziphilayo. USniedze ukhombe ukuthi i-diludine ivimbele umsebenzi [4] we-NADPH cytochrome C reductase ngokusobala ngemuva kokufunda ubudlelwano phakathi kwe-diludine ne-enzyme efanele ku-NADPH ethize yokudlulisa ama-electron ku-microsome yesibindi segundane. Ama-Odydents aphinde akhomba ukuthi i-diludine yayihlobene [4] nohlelo lwe-composite oxidase kanye ne-microsomal enzyme ehlobene ne-NADPH; futhi indlela yokusebenza kwe-diludine ngemva kokungena esilwaneni ukudlala indima yokumelana ne-oxidation nokuvikela ulwelwesi lwezinto eziphilayo [8] ngokuvimbela umsebenzi we-enzyme ye-NADPH yokudlulisa ama-electron ye-microsome nokuvimbela inqubo ye-peroxidation ye-lipid compound. Umphumela wokuhlolwa ukhombe ukuthi umsebenzi wokuvikela we-diludine kulwelwesi lwezinto eziphilayo kusukela ekushintsheni komsebenzi we-SOD kuya ekushintsheni kwemisebenzi ye-GPT ne-GOT futhi wafakazela imiphumela yocwaningo lwe-Sniedze ne-Odydents.
Ireferensi
1 Zhou Kai, Zhou Mingjie, Qin Zhongzhi, njll. Ucwaningo nge-diludine yokuthuthukisa ukusebenza kokuzala kwezimvuJ. Utshani kanyeLi-ivestock 1994 (2): 16-17
2 Qu Hai, Lv Ye, Wang Baosheng, Umphumela we-diludine wengezwe ekudleni kwansuku zonke ezingeni lokukhulelwa kanye nekhwalithi yesidoda senyama kanogwaja.Ijenali kaJ. Chinese Yokufuya Onogwaja1994(6): 6-7
3 Chen Jufang, Yin Yuejin, Liu Wanhan, njll. Ukuhlolwa kokusetshenziswa okwandisiwe kwe-diludine njengesithasiselo sokudlaUcwaningo Lokuphakelayo1993 (3): 2-4
4 Zheng Xiaozhong, Li Kelu, Yue Wenbin, njll. Ingxoxo ngomphumela wokusetshenziswa kanye nendlela yokusebenza kwe-diludine njengomgqugquzeli wokukhula kwenkukhuUcwaningo Lokuphakelayo1995 (7): 12-13
5 Chen Jufang, Yin Yuejin, Liu Wanhan, njll. Ukuhlolwa kokusetshenziswa okwandisiwe kwe-diludine njengesithasiselo sokudlaUcwaningo Lokuphakelayo1993 (3): 2-5
6 I-Bao Erqing, i-Gao Baohua, Ukuhlolwa kwe-diludine yokondla uhlobo lwedada lasePekingUcwaningo Lokuphakelayo1992 (7): 7-8
7 Ukuhlolwa kwe-Qin Shangzhi kokuthuthukisa umkhiqizo wezinkukhu zenyama zohlobo lwenyama ngesikhathi sokuphela kokuzala ngokusebenzisa i-diludineIjenali ye-Guangxi Yokufuya Izilwane kanye Nemithi Yezilwane1993.9(2): 26-27
8 Dibner J Jl Lvey FJ Iphrotheni yesibindi kanye ne-amino acid metabolian enkukhu Isayensi Yezinkukhu1990.69(7): 1188-1194
9 Yue Wenbin, Zhang Jianhong, Zhao Peie, njll. Ucwaningo lokwengeza i-diludine kanye nokulungiselela i-Fe-Zn ekudleni kwansuku zonke kwezinkukhu ezizalelayoUkudla Nemfuyo1997, 18(7): 29-30
10 Mildner A na M, Steven D Clarke Ukuhlanganiswa kwamafutha e-porcine fatty acid synthase kwe-DNA ehambisanayo, ukusatshalaliswa kwezicubu ze-itsmRNA kanye nokucindezela ukubonakaliswa yi-somatotropin kanye neprotheni yokudla J Nutri 1991, 121 900
11 W alzon RL Smon C, M orishita T, et a I I-Fatty liver hemorrhagic syndrome ezinkukhu ezidla ngokweqile ukudla okuhlanzekile Imisebenzi ye-enzyme ekhethiwe kanye ne-histology yesibindi maqondana nodumo lwesibindi kanye nokusebenza kokuzalaIsayensi Yezinkukhu,1993 72(8): 1479-1491
12 Donaldson WE Ukuguqulwa kwamafutha emzimbeni ekusabeleni kwesibindi samachwane lapho edlaIsayensi Yezinkukhu. 1990, 69(7): 1183-1187
13 Ksiazk ieu icz J. K ontecka H, H ogcw sk i L Inothi nge-cholesterol yegazi njengesibonakaliso sokukhuluphala komzimba kumadadaIjenali yeSayensi Yezilwane Nezondlayo,1992, 1(3/4): 289- 294
Isikhathi sokuthunyelwe: Juni-07-2021
